RNA

Part:BBa_K3252033:Design

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


gRNAJ23115-dCas9handle


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled by ordering custom gene synthetic.


Source

This part sequence was got from this reference.

Bikard, D., Jiang, W., Samai, P, Hochschild, A., Zhang, F., Marraffini, L. A., (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41(15):7429-37

Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283

References