RNA
Part:BBa_K3252033:Design
Designed by: Alfin Mohammad Abdillah Group: iGEM19_ITB_Indonesia (2019-10-21)
gRNAJ23115-dCas9handle
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was assembled by ordering custom gene synthetic.
Source
This part sequence was got from this reference.
Bikard, D., Jiang, W., Samai, P, Hochschild, A., Zhang, F., Marraffini, L. A., (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41(15):7429-37
Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283